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Effects of aging on hippocampal microglia of 3×Tg-AD and Non-Tg mice. (A) Representative Western blots for Iba1 and <t>CD11b/c</t> and (B , C) densitometric analyses normalized to β-actin loading control. Results are expressed as means ± SEM of percentage of controls (6-month-old/Non-Tg) (N = 3, in triplicate). Statistical analysis was performed by two-way ANOVA followed by Bonferroni’s post hoc test ( # p < 0.05, 6-month-old/3×Tg-AD vs 6-month-old/Non-Tg).
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Effects of aging on hippocampal microglia of 3×Tg-AD and Non-Tg mice. (A) Representative Western blots for Iba1 and CD11b/c and (B , C) densitometric analyses normalized to β-actin loading control. Results are expressed as means ± SEM of percentage of controls (6-month-old/Non-Tg) (N = 3, in triplicate). Statistical analysis was performed by two-way ANOVA followed by Bonferroni’s post hoc test ( # p < 0.05, 6-month-old/3×Tg-AD vs 6-month-old/Non-Tg).

Journal: Frontiers in Pharmacology

Article Title: Astrocyte Function Is Affected by Aging and Not Alzheimer’s Disease: A Preliminary Investigation in Hippocampi of 3xTg-AD Mice

doi: 10.3389/fphar.2019.00644

Figure Lengend Snippet: Effects of aging on hippocampal microglia of 3×Tg-AD and Non-Tg mice. (A) Representative Western blots for Iba1 and CD11b/c and (B , C) densitometric analyses normalized to β-actin loading control. Results are expressed as means ± SEM of percentage of controls (6-month-old/Non-Tg) (N = 3, in triplicate). Statistical analysis was performed by two-way ANOVA followed by Bonferroni’s post hoc test ( # p < 0.05, 6-month-old/3×Tg-AD vs 6-month-old/Non-Tg).

Article Snippet: Membranes were then incubated overnight with one of the following primary antibodies: rabbit anti-GFAP (1:25,000; Abcam, UK), rabbit anti-S100B (1:1,000; Novus Biological, CO), rabbit anti-Iba1 (1:1,000; Abcam), rabbit anti-CD11b/c (1:1,000; Bioss, MA), mouse anti-CX43 (1:500; EMD Millipore, MA), mouse anti-AQP4 (1:500; Santa Cruz, TX), rabbit anti-BDNF (1:1,000; Abcam), mouse anti-β-amyloid (1:200; Millipore, Germany), rabbit anti-p[Ser396]tau (1:1,000; Thermo Fisher Scientific, MA).

Techniques: Western Blot